catalytic activity:S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing 5-methylcytosine.,function:RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Not able to modify tRNAs at positions 48 or 49. May act downstream of Myc to regulate epidermal cell growth and proliferation.,PTM:Phosphorylated at Ser-139 by Aurora-B/STK12 during mitosis, leading to abolish methyltransferase activity and the interaction with NPM1.,similarity:Belongs to the methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily.,subcellular location:Concentrated in the nucleolus during interphase and distributed in the perichromosome and cytoplasm during mitosis.,subunit:Interacts with NPM1 and NCL during interphase; interaction is disrupted following phosphorylation at Ser-139.,
Nucleus, nucleolus . Cytoplasm . Mitochondrion . Cytoplasm, cytoskeleton, spindle . Secreted, extracellular exosome . Concentrated in the nucleolus during interphase and translocates to the spindle during mitosis as an RNA-protein complex that includes 18S ribosomal RNA (PubMed:19596847). In testis, localizes to the chromatoid body (By similarity). .
Expressed in adult and fetal brain and in lymphoblastoid cells.
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