LARP7 rabbit pAb

RNA-binding protein that specifically binds distinct small nuclear RNA (snRNAs) and regulates their processing and function . Specifically binds the 7SK snRNA (7SK RNA) and acts as a core component of the 7SK ribonucleoprotein (RNP) complex, thereby acting as a negative regulator of transcription elongation by RNA polymerase II . The 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation . The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC) . LARP7 specifically binds to the highly conserved 3'-terminal U-rich stretch of 7SK RNA; on stimulation, remains associated with 7SK RNA, whereas P-TEFb is released from the complex . LARP7 also acts as a regulator of mRNA splicing fidelity by promoting U6 snRNA processing . Specifically binds U6 snRNAs and associates with a subset of box C/D RNP complexes: promotes U6 snRNA 2'-O-methylation by facilitating U6 snRNA loading into box C/D RNP complexes . U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity . Binds U6 snRNAs with a 5'-CAGGG-3' sequence motif . U6 snRNA processing is required for spermatogenesis (By similarity).