catalytic activity:ATP + an acid + CoA = AMP + diphosphate + an acyl-CoA.,cofactor:Magnesium or manganese.,enzyme regulation:Activated by monovalent cations, such as potassium, rubidium or ammonium.,function:Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro).,similarity:Belongs to the ATP-dependent AMP-binding enzyme family.,subunit:Monomer.,tissue specificity:Detected in liver.,
Mitochondrion .
Detected in liver.
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