catalytic activity:Guanosine 3',5'-cyclic phosphate + H(2)O = guanosine 5'-phosphate.,cofactor:Divalent cations. Zinc ions yields maximum activity. Manganese, magnesium and cobalt also support catalysis but at much higher concentrations.,domain:Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.,enzyme regulation:Sildenafil (Viagra) is a highly selective and potent inhibitor of PDE5A and is effective in the treatment of penile erectile dysfunction. Also inhibited by zaprinast.,function:Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.,pathway:Purine metabolism; cGMP degradation; GMP from cGMP: step 1/1.,PTM:Phosphorylation is regulated by binding of cGMP to the two allosteric sites.,similarity:Belongs to the cyclic nucleotide phosphodiesterase family.,similarity:Contains 2 GAF domains.,tissue specificity:Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.,
cytosol,
Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
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